From Gallus Immunotech (Fergus, ON, Canada)

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Advantages of Avian IgY

There are several advantages to choosing chickens, rather than rabbit or goats to produce your polyclonal antibodies.

  • Chickens are not mammals and therefore are more apt to make high-avidity antibodies to mammalian antigens (especially highly conserved mammalian proteins).

  • To our knowledge, it is the most humane way to produce polyclonal antibodies. There is no need to bleed the chicken. Simply collect the eggs. Our hens are kept in flocks of six in coops that have outside runs. Our unique egg identification system allows our chickens to live freely.

  • A single chicken can produce an enormous amount of antibody, up to 3 grams of IgY per month, which is 10-20 times the amount of a rabbit. Furthermore, compared to rabbits, chickens produce antibody much quicker—high-titre antibody is available from eggs as early as day 25. Gallus Immunotech's custom IgY production services will deliver 0.5 gram of purified IgY to you within 50-60 days.

  • Fc region of chicken IgY is sufficiently different from mammalian IgG: Reduces background by not binding to mammalian rheumatoid factors or other naturally occurring anti-mammalian antibodies (e.g. HAMA)

  • IgY vs IgG
  • Does not activate mammalian complement systems

  • Does not bind to mammalian Fc receptors

  • Does not bind to standard immunoglobulin-binding proteins such as protein A (Staphylococcus aureus), protein G (Streptococcus sp.), or protein L (Peptostreptococcus magnus)

  • By having the immunoglobulin Y (IgY) packaged conveniently in eggs, eggs can be stored over a long period of time and the IgY purified from the eggs of desired titre/avidity.

  • It is cheaper to feed and house chickens than rabbits.

  • IgY is a stable antibody sharing the following characteristics with mammalian IgG:

    » Divalent

    » Degraded by papain to yield divalent Fab fragment

    » May be enzyme-labeled, biotinylated and gold–labeled by standard procedures


  • Gallus Immunotech's Eggspertise

    Gallus Immunotech was co-founded by Dr. Kelsye Coulter, a trained immunologist, in 1994. They were one of the first North American companies to specialize in chicken antibodies, at a time when most researchers in the diagnostic and pharmaceutical industries had never heard of IgY.

    Beginning as a 'fledgling' in this new commercial field, Gallus Immunotech has matured into a business with world-class IgY expertise and a world-wide customer base.

    Early on, Gallus Immunotech commercialized their proprietary IgY EggPress Purification Kit in collaboration with a Canadian scientist whose PhD thesis subject was the efficient purification of IgY from egg yolk. Their purification kit boasts a high IgY yield (app. 6-7 mg IgY/gram yolk) and is very easy to use.

    IgY EggPress Purification Kit
    Gallus Immunotech strives to deliver high quality chicken antibodies in milligram quantities for the researcher or up to kilogram quantities for diagnostic or industrial use. All laboratory work is performed using standard operating procedures to assure the highest quality of our products. With their experienced scientists on hand to discuss small or large projects, they pride themselves in providing personable service and attention to detail.

    Gallus Immunotech's products are now made available to Malaysia research communities by MBIO.

    Get a quote for IgY EggPress Purification Kit

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    Chickens are the Humane Alternative

    An important aspect of Gallus Immunotech's business has been to promote the humane alternative of using chickens to produce polyclonal antibodies. To this end, Gallus Immunotech houses their chickens in very pleasant and as natural as possible surroundings. Their chickens enjoy nesting boxes, roosting sticks, dry baths (woodchips or sand) and outside runs when the weather permits. Their animal facilities are inspected and accredited under the Ontario Animals for Research Act licence #0028, which is overseen by the Ontario Ministry of Agriculture, Food and Rural Affairs.

    hens
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