Aflatoxin Fungal Toxin Detection Kits

aflatoxin detection and mycotoxin detection


About Aflatoxin, a fungal toxin

Aflatoxins are produced as secondary metabolites by Aspergillus flavus and aspergillus parasiticus. The fungi themselves are ubiquitous in nature and will grow and produce aflatoxins on animal and human food commodities under certain conditions of harvesting and storage. Aflatoxins, in particular Aflatoxin B1, are potent liver carcinogens(1) and in sufficiently high concentrations will cause acute liver failure and death (aflatoxicosis). The presence of aflatoxins in human and animal food commodities is stringently monitored in the developed world but due to limited technological infrastructure, exposure to aflatoxins may be more prevalent in developing countries(2).

When cows are fed contaminated feed, Aflatoxin B1 is converted to Aflatoxin M1, which is subsequently secreted in the milk of lactating cows. Aflatoxin M1 is quite stable towards normal milk processing methods such as pasteurization, and if present in raw milk, it may persist into final products for human consumption.

After ingestion of alfatoxin contaminated food by human, Aflatoxin B1 is rapidly metabolized by the liver to a variety of by-products which are then excreted in one form or another. In several epidemiological studies attempting to correlate aflatoxin consumption with the occurrence of liver cancer, it has been shown that the appearance of Aflatoxin M1 in urine provides a good indicator of aflatoxin B1 consumption and that about 2% of ingested aflatoxin B1 appears as aflatoxin M1 in urine (3).

REFERENCES
1) Wogan, Gerald N., Aflatoxins as Risk Factors for Hepatocellular Carcinoma in Humans. Cancer Research 1992; 52: 2114s-2118s

2) Williams, Jonathan, et. al., Human Aflatoxicosis in Developing Countries: A review of Toxicology, Exposure, Potential Health Consequences, and Interventions. American Journal Clinical Nutrition 2004; 80: 1106-22

3) Zhu, Jia-qi, et. al., Correlation of Dietary Aflatoxin B1 Levels with Excretion of Aflatoxin M1 in Human Urine. Cancer Research 1987; 47: 1848-1852

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Mycotoxin Testing Kits from Helica Biosystems (California, USA)

  • Rapid and highly sensitive kits

  • Results in 30 – 90 minutes

  • Easy sample extraction

  • No matrix effect

  • Cost-effective and user-friendly tests

  • Break-away wells allow low-volume testing

  • Standards and conjugate preserved with proprietary stabilizers

  • One year stability and reproducibility of results

  • Color-coded reagents in convenient, easy to use vials

  • Easy to follow instructions


List of Aflatoxin Kits from Helica Biosystem

Product Cat # Sample Type Kit Description
Aflatoxin B1 ELISA (Rapid Format) 941BAFL01-96 Grains, cereals, nuts, cottonseeds, animal feed. A competitive enzyme-linked immunoassay intended for the quantitative detection of Aflatoxin B1 in grains, nuts, cottonseeds, cereals and other commodities including animal feed. With assay time of under 30 minutes.
Aflatoxin B1 ELISA (Low Matrix) 981BAFL01-96 Baby formulas, oils, nuts A competitive enzyme-linked immunoassay intended for the quantitative detection of Aflatoxin B1 in toddler and infant formulas, rice cereal, corn, wheat, silage, peanut, soy sauce, soy bean, sesame, safflower, vegetable oil, corn oil, peanut oil, and animal feed. It is designed for special application in commodities difficult to assess by the rapid format due to matrix interference with assay time of 75 minutes.
Aflatoxin M1 2000 ELISA (Rapid Format) 961AFL01M-US Milk and dairy products For the quantitative detection of Aflatoxin M1 in milk, reconstituted milk powders and cheese. With assay time of under 35 minutes. Detection range: 0 - 2000 pg/mL(ppt)
Aflatoxin M1 ELISA High Sensitivity 961AFLM01M-96 Milk and dairy products For the quantitative detection of Aflatoxin M1 in milk, reconstituted milk powders and cheese. With assay time of 2 hr. 30 min. Detection range: 0 - 100 pg/mL(ppt)
Aflatoxin M1 ELISA In Urine 991AFLM01U-96 Urine For the quantitative detection of Aflatoxin M1 in urine samples.
Total Aflatoxin Assay 941AFL01M-96 Grains, cereals, nuts, animal feed A competitive enzyme-linked immunoassay intended for the quantitative detection of Aflatoxin B1, B2, G1 and G2 in grains, nuts, cottonseeds, cereals and other commodities including animal feeds.
Total Aflatoxin Assay (Low Matrix) 981AFL01LM-96 Grains and cereal, silage, nuts, spices, animal feed A competitive enzyme-linked immunoassay intended for the quantitative detection of Aflatoxin B1, B2, G1 and G2 in grains, nuts, cottonseeds, cereals and other commodities including animal feeds. It is designed for special application in commodities difficult to assess by the rapid format due to matrix interference.

Visit Helica Biosystems's website to view all the available mycotoxin assays.

Link to Helica Biosystems

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