Muta-ChromoPlate™ Reverse Mutation Assay (Ames Test)

 EBPI's Muta-ChromoPlate Ames Test Kit

• Principle of Ames Test
• About our Muta-ChromoPlate™
• Application of Muta-ChromoPlate™

Principle of Ames Test

ames test kit in malaysia

The Muta-ChromoPlate™ kit is based on the most generally used and obacterial reverse-mutation test, known as the 'Ames Test' (Ames et al., 1975, Mutation Research 31:347).

The test employs a mutant strain, or several strains, of Salmonella typhimurium, carrying mutation(s) in the operon coding for histidine biosynthesis. When these bacteria are exposed to mutagenic agents, under certain conditions, reverse mutation from amino acid (histidine) auxotrophy to prototrophy occurs.

Traditionally, reverse-mutation assays have been performed using agar plates. An alternate assay performed entirely in liquid culture is the 'Fluctuation Test' based on multiple yes/no colour endpoints known as the Muta-ChromoPlate kit.

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About EBPI's Muta-ChromoPlate™

muta-chromo ames testThe Muta-ChromoPlate™ is a 96-well microplate version of the salmonella typhimurium 'Ames Test,' used for detection of mutagenic activity.

Developed to test mutagenic materials in water soluble extracts of sediment, air, chemicals, food components, cosmetics, waste waters, portable waters and any other material that can be solubilized or placed into micro suspension in water, such that the material being tested can be taken up by the test strain.

The Muta-ChromoPlate™ provides a clear colour endpoint. Reagents, cultures and other consumable components are supplied ready-to-use in a non-specialized laboratory.

muta-chromoplate ames test result

Colour endpoints of the Muta-ChromoPlate™:

Purple- No Mutation

Yellow- Reverse Mutation Occurred

The Muta-ChromoPlate™ kit is generally more sensitive than the pour-plate assay, because it allows testing of higher concentrations of sample (up to 75% v/v). The assay procedure is simple and requires minimal training. Consumable components are provided with ready-to-use and step-by-step instructions. "Instructions for Use" are provided with the basic kit. The only equipment required are a 37 degree Celsius incubator and a single and a multi-channel micropipettor.

S9 and Activation of Pro-Mutagens

S9 is a crude liver enzyme extract that can, under certain conditions, convert materials without any genotoxic activity to active genotoxic entities. The chemical process involved may be different for different materials. In addition, the lifetime of the activated moieties is extremely variable: some may be extremely short-lived. This is the reason for incubating the S-9 with the bacteria and the tested material at the same time during the assays.

5051 Muta-ChromoPlate Basic Kit

Developed to test mutagenic materials in water soluble extracts of sediment, air, chemicals, food components, cosmetics, waste waters, portable waters and any other material that can be solubilized or placed into micro suspension in water, such that the material being tested can be taken up by the test strain.
  • Includes 1 Bacterial Strain, growth media and control (TA100 default unless otherwise requested)
  • Reagents and plastics for 12 plates (including reagents boats, micro-plates, filter unit)

Bacterial Strains which can be included in the Muta-ChromoPlate kit are as follows:

TA100, TA98, TA1535, TA97a, WP2 uvrA, WP2 uvrA pkm101

B5051 Muta-ChromoPlate Bacterial Strain Kit

  • Includes 2 Bacterial Strains, growth media and controls (TA100 and TA98 are default strains unless otherwise requested)
  • Reagents and plastics for 24 plates (including reagents boats, micro-plates, filter units)

Bacterial Strains which can be included in the Muta-ChromoPlate kit are as follows:

TA100, TA98, TA1535, TA97a, WP2 uvrA, WP2 uvrA pkm101

Item Catalog # Description Download
Muta-ChromoPlate Basic Kit 5051 Contains 1 bacterial, reagents, controls and plastics Muta-ChromoPlate Presentation
Muta-ChromoPlate Bacterial Strain Kit B5051 Contains 2 bacterial, reagents, controls and plastics Muta-ChromoPlate Flow Chart
S9 Activation Enzymes 5051S9 For metabolic activation Excel Template Use Instructions

 

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Applications of Muta-ChromoPlate™

  • Testing of pharmaceuticals for mutagenic activity
  • Testing of industrial effluents for presence of possible mutagenic compounds
  • Screening of municipal discharges for possible routine presence or spills of mutagenic compounds
  • Screening of surface and/or groundwater for mutagenic residues
  • Screening of potable water supplies for the presence of chemicals with mutagenic potential
  • Screening of water soluble air pollutants for mutagenic agents
  • Evaluation of pure or complexed raw mixtures for potential mutagenicity
  • A convenient and easy to use teaching tool for university and college laboratories

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