Cyanase TMNuclease: Your alternative for Benzonase®

cyanase

For fast and efficient removal of nucleic acid contamination during protein purification
Benzonase = trademark of Merck KGaA, Cyanase = trademark of RiboSolutions, Inc.


About Cyanase TMNuclease

Cyanase is a cloned highly active non-Serratia based non-specific endonuclease that degrades single and double stranded DNA and RNA in as little as 1 minute.

• Fastest Nuclease on the market
• Broad pH range
• Active in higher salt concentrations (up to 100 mM NaCl)
• Unaffected by lysozyme, detergents or urea, DTT 
• Easily inactivated and removed 
• Unsurpassed stability, can be stored up to 1 year at room temperature

Cyanase comparison

cyanase

Click to enlarge image

The figure above demonstrates the superior activity of Cyanase™ versus leading market nucleases. 3 ug of Lambda DNA was incubated with various amounts of Cyanase™ and competitor enzymes for 1 minute at 37°C in their manufacturer recommended optimal buffers. Each reaction was then run on a 1% agarose gel. Only the Cyanase lanes demonstrate complete degradation of the target band at either unit amount.


Product Ordering

Products Cat # Size Description
Cyanase Nuclease 10,000 U (50 U/µl) 1000 10,000 U Benzonase nuclease alternative: Cyanase nuclease. Fastest and most stable nuclease on the planet. Supplied with 1 ml of 1 M MnSO4. Store at -20°C long term.
Cyanase Nuclease 25,000 U (50 U/µl) 1005 25,000 U Benzonase nuclease alternative Cyanase nuclease. Fastest and most stable nuclease on the planet. Supplied with 1 ml of 1 M MnSO4. Store at -20°C for long term.
Cyanase Nuclease 100,000 U (50 U/µl) 1030 100,000 U For the quantitative detection of Aflatoxin M1 in milk, reconstituted milk powders and cheese. With assay time of under 35 minutes. Detection range: 0 - 2000 pg/mL(ppt)
Nucleic Acid Removal Kit 1050 10,000 U Cyanase, 1 mL Inactivation Resin, and 1 mL of 1 M MnSO4 Nucleic acid removal all in one. Cyanase Nuclease and inactivation resin together in one kit. Designed to quickly remove all contaminating nucleic acids via the Cyanase nuclease and then easily remove the Cyanase for downstream applications via the inactivation resin. Removal is based on a highly specific affinity to the Cyanase only. No effect on other proteins or reaction conditions. Neither Benzonase nor DNase I have any removal processes this specific or effective.
1 mL Cyanase Inactivation Resin (50% slurry, 0.5 mL beads) 1010 1mL Supplied as 1 ml of a 50% slurry in 50 mM Tris pH 8.0, 2 mM MgSO4. Store at +4°C.
5 mL Cyanase Inactivation Resin (50% slurry, 2.5 mL beads) 1020 5mL Supplied as 1 ml of a 50% slurry in 50 mM Tris pH 8.0, 2 mM MgSO4. Store at +4°C.
Nucleic Acid Removal Kit Cartridges 1055 20 Cartridges with 50 µL of Resin, 20 Collection Tubes with Caps, 10,000 U of Cyanase™ Nuclease, 1 mL of 1 M MnSO4. Fast and easy removal of Cyanase in high throughput applications where addition of resin is undesirable.

Visit Ribosolution's website to view all the available cyanase-based kits.

Link to RiboSolutions

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