Diaclone ELISpot Kits and ELISpot Sets

Diaclone ELISpot by MBIO

• Introduction of Enzyome-Linked Immunospot Assays (ELISpot)
• ELISpot Applications
• Why use Diaclone's ELISpot kits?
• Diaclone ELISpots: Formats Available
• List of Diaclone ELISpot kits
• Resources

Introduction of Enzyme-Linked Immunospot Assays (ELISpot)

Diaclone ELISpots (Enzyme-Linked Immunospot Assays) are highly specific immunoassays for the analysis of cytokine and other soluble molecule production and secretion from T-cells at a single cell level in conditions closely comparable to the in-vivo environment with minimal cell manipulation. Utilising sandwich immuno-enzyme technology, Diaclone ELISpot and Dual ELISpot assays can detect both secreted cytokines and measure the frequency of single cells that simultaneously produce multiple cytokines or other effector molecules.

As well as single analyte detection Diaclone Dual ELISpot (Enzymatic detection) and Dual Fluorospot (Fluorescent detection) Sets are available for multiple analyte detection. Dual sets enable the simultaneous detection of cells secreting either individual analyte or cells co-secreting both analytes

Diaclone's ELISpot Illustration by MBIOSCIENCE

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ELISpot Applications

Monitoring the immune responses of patients treated with immunotherapy is essential to evaluate the efficacy of treatment and correlate clinical responses to Cytotoxic T Lymphocyte responses.

ELISpot assays are specific, accurate, sensitive, precise and robust. This bioassay is now recognized as the reference method to evaluate vaccine efficacy in clinical trials and has an ever expanding role in many immunology related research clinical fields.

Vaccine Development

  • Assessment of vaccine efficacy to induce potent T-cell responses
  • Antigen specific cytokine responses
  • Novel vaccine adjuvants

Cancer

  • Evaluation of the efficacy of novel Cancer Immunotherapies
  • Investigation in changes in T-Cell populations during disease progression

Allergy

  • Measurement of Th1 / Th2 cell responses
  • Evaluation of the efficacy of novel allergen specific Immunotherapies

Autoimmune Disease

  • Secretory profile of T-Cells under conditions of autoimmunity

Transplantation

  • Tool for prediciting post transplant rejection
  • Quantification of frequency and cytokine profile of circulating donor reactive T-cells

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Why use Diaclone's ELISpot kits?

Diaclone ELISpot are highly specific immunoassays for the analysis of cytokine and other soluble molecule production and secretion from individual cells in conditions closely comparable to the in-vivo environment with minimal cell manipulation.

  • High sensitivity - detect a single secreting cell out of 100,000
  • Applications in multiple fields including Immune-monitoring in vaccine development, cancer, autoimmunity & transplantation
  • Flexibility: Antibody pairs, ELISpot Sets (with or without plates), Pre-coated Kits
  • Dual ELISpot & Fluorospot kits for multiple analyte detection
  • Discover Diaclone’ - trial sizes available upon request(1x96)
  • Manufactured to ISO9001:2008 standards
diaclone ELISpot wells from MBIOSCIENCE

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Diaclone's ELISpot: Formats Available

  • Diaclone ELISpot Kits: Pre-coated PVDF plate(s), Detection antibody, Alkaline Phosphatase conjugate, BSA and BCIP/NBT ready-to-use substrate buffer
  • Diaclone ELISpot Sets: Capture and Detection antibody, Alkaline Phosphatase conjugate, BSA and BCIP/NBT ready-to-use substrate buffer.
  • Diaclone ELISpot Pair: Capture and Detection Antibodies.
  • Dual ELISpot Sets: Capture and Detection antibodies for two analytes, Alkaline phosphatase conjugate, blocking reagent, ready-to-use BCIP/ NBT substrate buffer, Peroxydase conjugate, BSA, ready-to-use AEC substrate buffer.
  • Dual Fluorospot Sets: Capture antibody for two analytes, FITC-conjugated Detection antibody for cytokine 1, anti-FITC antibody green fluorescence conjugate, biotinylated Detection antibody for cytokine 2, streptavidin-phycoerythrin conjugate, BSA.

 

Complete your Catalogue Number by choosing your format:
  Format Cat # (see listings below)
ELISpot Kits 1x96 Discovery xxx.xxx.001PC
5x96 xxx.xxx.005PC
ELISpot Sets
Dual ELISpot Sets
Dual Flurospot Sets
1x96 Discovery xxx.xxx.001
5x96 xxx.xxx.005
10x96 xxx.xxx.010
15x96 xxx.xxx.015
20x96 xxx.xxx.020
Note: Discovery size not applicable to 6xx.xxx.xxx products


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List of Diaclone ELISpot Kits

ELISpot
Analytes Species ELISpot Kit ELISpot SetELISpot-Pair
Human Kits
CD178 Human 856.091.xxx 856.091.xxx869.080.010
Granzyme B Human 856.131.xxx 856.131.xxx869.130.010
IFNγ Human 856.051.xxx 856.051.xxx869.060.010

IL-1β Human 856.101.xxx 856.101.xxx869.100.010

IL-2 Human 856.001.xxx 856.001.xxx869.000.010

IL-4 Human 856.011.xxx 856.011.xxx869.010.010
IL-5 Human 856.111.xxx 856.111.xxx869.110.010
IL-6 Human 856.021.xxx 856.021.xxx869.020.010
IL-10 Human 856.031.xxx 856.031.xxx869.030.010
IL-12p70 Human 856.081.xxx 856.081.xxx869.040.010
IL-13 Human 856.121.xxx 856.121.xxx869.120.010
IL-17A Human 856.151.xxx 856.151.xxx869.150.010
IL-17F Human 856.161.xxx 856.161.xxx869.160.010
IL-17A/F Human 856.171.xxx 856.171.xxx869.170.010
Perforin Human 856.141.xxx 856.141.xxx869.140.010
TNFα Human 856.041.xxx 856.041.xxx869.050.010
Murine Kits
IFNγ Murine 862.031.xxx 862.031.xxx870.050.010
IL-2 Murine 862.001.xxx 862.001.xxx870.010.010
Rat Kits
IFNγ Rat 867.011.xxx 867.011.xxx871.020.010
TNFα Rat 867.021.xxx 867.021.xxx871.030.010

Note: ELISpot Sets are provided without plates, if you wish to order non-sterile plates add a P to the catalogue number and for sterile plates add an S.

Contact Team MBIO for assistance in kit selection.

Dual ELISpot
Analyte 1 Analyte 2 SPECIES Dual ELISpot SetDual Flurospot Set
IFNγ Perforin Human 874.070.xxx
IFNγ Granzyme B Human 874.060.xxx874.062.xxx
IFNγ IL-2 Human 874.040.xxx874.040.xxx
IFNγ IL-4 Human 874.010.xxx874.002.xxx
IFNγ IL-5 Human 874.020.xxx874.032.xxx
IFNγ IL-10 Human 874.000.xxx874.012.xxx
IFNγ IL-17 Human 874.080.xxx874.082.xxx
IL-10 IL-2 Human 874.050.xxx874.052.xxx
IL-10 IL-4 Human 874.030.xxx874.022.xxx
IL-17A IL-4 Human 874.092.xxx

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Resources

 

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