World's First ELISA Based Endotoxin Detection System - EndoLISA®

hyglos endotoxin detection

About Endotoxins (LPS)

Endotoxins are natural compounds found in the outer cell membrane of Gram-negative bacteria and impacts over 30 biological activities. It has been shown that endotoxins can affect research results such as tissue culture and can be toxic to man/animals causing fevers if injected. Hence, the detection and removal of endotoxin is very important for ongoing research. However, current LAL methods of detecting endotoxin have been shown to lack robustness, reproducibility and flexibility for running multiple samples as well as using an ingredient derived from horseshoe crabs.

horseshoe crab for LAL test

Figure (1): Each year, half a million horseshoe crabs are captured and bled alive for the production of LAL test kits.

EndoLISA®: a novel and reliable method for endotoxin detection

MBIO offers the EndoLISA® for the efficient and reliable determination of endotoxin (LPS) levels from Hyglos. The EndoLISA® is the world's first endotoxin detection system based on ELISA-technology. This ELISA utilises a Hyglos developed and optimised lipopolysaccharide (LPS) specific phage-derived protein in combination with optimal sample conditions, in order to specifically cover the entire substance group of LPS (endotoxin).

Benefits of Using the EndoLISA® The EndoLISA® offers numerous advantages over typical LAL assays:

  • World's first endotoxin detection system based on ELISA-technology

  • Overcomes limitations of existing methods, such as the need for substantial dilution

  • Reduced matrix effects due to integrated washing step

  • Robust assay

  • Excellent reproducibility

  • Wide measurement range - 0.05 - 500 EU/ml

  • Wide pH range - 4 - 9

  • Wide measurement range - 0.05 - 500 EU/ml

  • No interference in high salt conditions e.g. NaCl, GdnHCl

  • Flexible testing format - microtiter plate for multiple samples

  • Animal free - no animal products are used to generate this kit. Specifically, this kit does not use horseshoe crab derivatives
  • endolisa for endotoxin test and detection

    Figure (2): How it works? The phage protein is pre-coated to the wells in the EndoLISA® microtiter plate and as the sample is added to the well, the endotoxin (LPS) in the sample is bound to the phage protein. Any sample matrix with potentially interfering components is then completely removed by a washing step. Therefore, the subsequent detection by recombinant Factor C (rFC) and a fluorescence substrate is left unaffected by inhibitors, facilitating a reliable quantification of endotoxin in the sample

    Download EndoLISA flyer here (PDF, 3.7MB)»

    Get a quote

    Back to top

    EndoLISA® ELISA Video

    EndoLISA® References

    The EndoLISA® has been referenced in numerous publications including:

    Dhanasooraj D, Kumar RA et Mundayoor S. Vaccine delivery system for tuberculosis based on nano-sized hepatitis B virus core protein particles.Int J Nanomedicine. 2013;8:835-43. doi: 10.2147/IJN.S40238. Epub 2013 Feb 25.
    Toussaint M et al. Increased hypoxia-inducible factor 1a expression in lung cells of horses with recurrent airway obstruction. BMC Veterinary Research May 2012, 8:64.

    Download list of customer publication here (PDF, 147kb)»

    Back to top

    Endotoxin Detection With Non-Complex Sample & Buffer Conditions - EndoZyme®

    This EndoLISA® is suited for complex samples and buffer conditions. For non-complex sample and buffer conditions, we recommend that EndoZyme®is used.

    Back to top