Immunotoxicity ELISA kits from Life Diagnostics

mbio immunotoxicity ELISA kits


About Immunotoxicity

Anti-KLH, anti-SRBC, anti-tetanus toxoid, anti-DNP and anti-TNP IgM and IgG are routinely used as biomarkers of immunotoxicity. Life Diagnostics manufactures ELISA kits for these important biomarkers in mice, rats, chickens, and monkeys. In preclinical studies, animals are immunized with an immunogen such as KLH while being dosed with a drug candidate. Serum is collected at either 7 or ~ 21 days and the levels of anti-KLH IgM and IgG are determined and compared with those in a control group that was not exposed to the drug. A decrease in anti-KLH levels in the treatment group provides evidence of immunosuppression.

Immunotoxicity ELISA Kits from Life Diagnostics(Pennsylvania, USA)

Our rat and monkey anti-KLH IgG ELISA kits have recently been evaluated and gained unqualified approval in a respected independent laboratory (Validation of an ELISA assay for quantification of anti-KLH IgG in rat and monkey sera. CIT, Evreux, France. Armelle N. Guyen, Sandrine Arthaud, Phillipe Ancian and Roy Forster. Toxicology Letters, Volume 180, Supplement 1, 5 October 2008, Page S211).

klh

Publications describing the use of LDI Immunotoxicity kits

  1. Guyen AN, Arthaud S, Ancian P and Foster R. Validation of an ELISA assay for quantification of anti-KLH IgG in rat and monkey sera. Toxicology Letters (Abstracts) 180S: S211/I11 (2008)
  2. Betz BC, Jordan-Williams KL, Wang C, Kang SG, Liao J, Logan MR, Kim CH and Taparowsky EJ. Batf coordinates multiple aspects of B and T cell function required for normal antibody responses. J. Exp. Med. 207(5):933-942 (2011) [Mouse anti-SRBC IgG & IgM] http://jem.rupress.org/content/207/5/933.full.pdf+html 
  3. Schaffer BS, Grayson MH and Wortham JM, et al. Immune competency of a hairless mouse strain for improved preclinical studies in genetically engineered mice. Mol. Cancer Ther. 9:2354-2364 (2010) [Mouse anti-KLH IgG & IgM] http://mct.aacrjournals.org/content/9/8/2354.full.pdf+html 
  4. Efanov A, Zanesi N, NazaryanN, Santanam U, Palmarchuck A Croce CM and Pekarsky Y. CD5+CD23+ leukemic cell populations in TCL1 transgenic mice show significantly increased proliferation and Akt phosphorylation. Leukemia 24:970-975 (2010) [Mouse anti-SRBC IgM]
  5. Nain S and Smits JEG. Pathological, immunological and biochemical markers of subchronic arsenic toxicity in rats. Environmental Toxicology. (2010)http://onlinelibrary.wiley.com/doi/10.1002/tox.20635/abstract  [Rat anti-KLH IgG & IgM]
  6. Zhou X, Xiang Y, Ding, X and Garrard WT. Anew hypersensitive site, HS10, and the enhancers, E3′ and Ed, differentially regulate Igk gene expression. J. Immunol. 188:2722-2732 (2012) [Mouse anti-KLH IgG & IgM]
  7. Sah S and Smits J. Dietary selenium fortification: a potential solution to chronic arsenic toxicity. Toxicological & Environmental Chemistry. 94(7): 1453-1465 (2012) [Rat anti-KLH IgG]
  8. Anderson SE, Franko J, Anderson KL, Munson AE, Lukomska E, Meade BJ. Immunotoxicity and allergic potential induced by topical application of dimethyl carbonate (DMC) in a murine model. J Immunotoxicol. [Epub ahead of print] (2012) [Mouse anti-SRBC IgM]
  9. Aulí M, et al. Multiparametric immunotoxicity screening in mice during early drug development. Toxicol Lett. [Epub ahead of print] (2012) [Mouse anti-KLH IgM]
  10. Sah S and Smits J. Treating chronic arsenic toxicity with high selenium lentil diets. Toxicol Appl Pharmacol. doi: 10.1016/j.taap.2013.06.008. (2013) [Rat anti-KLH IgG]

List of Immunotoxicity ELISA Kits

Anti-KLH IgG & IgM ELISA Kits
ELISA Kit Kit Instructions Catalog #
Mouse Anti-KLH IgM Insert 4000-1
Mouse Anti-KLH IgG Insert 4010-1
Rat Anti-KLH IgM Insert 4000-2
Rat Anti-KLH IgM with Controls Insert 4000-2C
Rat Anti-KLH IgG Insert 4010-2
Rat Anti-KLH IgG with Controls Insert 4010-2C
Guinea Pig Anti-KLH IgG Insert 4010-3
Monkey Anti-KLH IgM (ng/ml) Insert 4000-4-N
Monkey Anti-KLH IgM (ng/ml) (For Export)* Insert 4000-4-INT
Monkey Anti-KLH IgG1 (ng/ml) Insert 4010-4-1
Monkey Anti-KLH IgG (ng/ml) (For Export)* Insert 4010-4-INT
*Kits 4000-4-INT and 4010-4-INT use standard material of non monkey origin, allowing for exportation without CITES documentation.

Anti-Sheep Red Blood Cell IgG & IgM ELISA Kits

ELISA Kit Kit Instructions Catalog #
Mouse Anti-SRBC IgM Insert 4200-1
Mouse Anti-SRBC IgG Insert 4210-1
Rat Anti-SRBC IgM Insert 4200-2
Rat Anti-SRBC IgG Insert 4210-2
Chicken Anti-SRBC IgM Insert 4200-5
Chicken Anti-SRBC IgG Insert 4210-5

Anti-Tetanus Toxoid IgG & IgM ELISA Kits

ELISA Kit Kit Instructions Catalog #
Rat Anti-Tetanus Toxoid IgM Insert 4300-2
Rat Anti-Tetanus Toxoid IgG Insert 4310-2
Monkey Anti-Tetanus Toxoid IgM (ng/ml) Insert 4300-4-N
Monkey Anti-Tetanus Toxoid IgG1 (ug/ml) Insert 4310-4-1

Anti-DNP IgG & IgM ELISA Kits

ELISA Kit Kit Instructions Catalog #
Mouse Anti-DNP IgM Insert 4500-1
Mouse Anti-DNP IgG Insert 4510-1
Rat Anti-DNP IgM Insert 4500-2
Rat Anti-DNP IgG Insert 4510-2

Anti-TNP IgG & IgM ELISA Kits

Mouse Anti-TNP IgM Insert 4600-1
Mouse Anti-TNP IgG Insert 4610-1

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