BioMedica's Lipid Peroxidation & Oxidative Stress ELISA

 Biomedica's Lipd Peroxidation & Oxidative Stress ELISA

• Scientific Relevane of oxLDLs
• Biomedica's Oxidative Stress Product Line
• Biomedica's MDA-oxLDL ELISA
• Biomedica's oLAB ELISA (Anti oxidized-LDL autoantibodies)
• Biomedica's OxyStat Assay- CE marked
• Resources

Scientific Relevance of oxLDLs

Oxidatively modified lipoproteins (oxLDLs) play an important role in the progression of atherosclerosis and coronary artery disease. Accumulation of oxLDL in macrophages and smooth muscle cells causes foam cell formation, an initial step in the disease. Low-density lipoprotein (LDL), the main carrier of plasma cholesterol, consists of a hydrophobic core and a surface monolayer of polar lipids and Apolipoprotein-B (ApoB). Oxidative stress and the consequent formation of free radicals lead to the peroxidation of ApoB. Malondialdehyde (MDA) has been identified as one of the major lipid peroxidation products of LDL, thus playing an important role in the LDL oxidation.

Malondialdehyde (MDA)

Oxidative damage to lipids in the cell membrane generates the reactive decomposition product malondialdehyde (MDA), which forms adducts with cell proteins.

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Biomedica's Oxidative Stress Product Line

Biomedica's oxidative stress product line covers biomarkers for risk stratification (OxyStat and MDA-oxidized LDL) and one biomarker to determine the severity of disease (oLAB - anti oxidized LDL autoantibodies).

Our ELISA assays include six calibrators and one to two controls in human serum matrix, thus allowing researchers to collect biologically reliable data.

 

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Biomedica's MDA-oxLDL ELISA

Malondialdehyde (MDA) has been identified as one of the major lipid peroxidation products of LDL, thus playing an important role in the LDL oxidation.

The Biomedica MDAoxLDL ELISA specifically detects MDA-modified ApoB in human serum and citrate-plasma.

Assay characteristics:

Cat.No.: BI-20022
Method: Sandwich ELISA, HRP/TMB, 12x8-well strips
Sample type: Serum, plasma (EDTA, heparin, citrate)
Standard range: 0-10 µg/ml (6 serum based standards) 
Standard points: 0/0.6/1.25/2.5/5/10 µg/ml
Control: 1 serum based control
Sample size: 20 µl / well
Incubation time: 1.5 h / 1.5 h / 30 min

Sensitivity:
LOD: 0.05 µg/ml (0 µg/ml + 3 SD)

Precision:
Intra-assay (n=3) ≤ 8%, Inter-assay (n=4) ≤ 7%

Values from apparently healthy individuals:
Median (serum, n=50): 263 mU/ml.
It is recommended to establish the normal range for each laboratory.

 

Download MDA-oxLDL ELISA's Protocol

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Biomedica's oLAB ELISA (Anti oxidized-LDL autoantibodies) - CE marked

Anti oxidized LDL autoantibodies. - CE marked - for IVD use in EU!

Autoantibodies against oxidatively modified LDL can be used as a parameter that consistently mirrors the occurrence of oxidation processes taking place in vivo. The Biomedica oLAB ELISA has been developed to detect human IgG autoantibodies against oxLDL in human serum. Elevated levels of autoantibodies against oxLDL have been detected in the blood stream of patients with coronary artery disease. Moreover, studies indicate a correlation between autoantibodies against oxLDL (oLAB) and the progression of carotid atherosclerosis. Increased serum concentrations of oLAB have also been described in various diseases such as pre-eclampsia and systemic lupus erythematosus. Decreased oLAB titers were observed during septicemia and myocardial infarction.

Assay characteristics:

Cat.No.: BI-20032
Method: Sandwich ELISA, HRP/TMB, 12x8-well strips
Sample type: Serum
Standard range: 0-1,200 mU/ml (6 standards)
Standard points: 37/75/150/300/600/1200 mU/ml
Controls: 2 controls
Sample size: 20 µl / well
Incubation time: 1.5 h / 30 min / 15 min

Sensitivity:
LOD: 48 mU/ml (37 mU/ml + 3 SD)

Precision:
Intra-assay (n=8) ≤ 4%, Inter-assay (n=5) ≤ 8%

Values from apparently healthy individuals:
Median (serum, n=50): 263 mU/ml.
It is recommended to establish the normal range for each laboratory.

 

Download oLAB ELISA's Protocol

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Biomedica's OxyStat Assay- CE marked

Quantitative determination of peroxides in biological fluids. - CE marked - for IVD use in EU.

Cells and tissues are sensitive to oxidative stress, caused by the formation of free radicals. If not deactivated by antioxidants, organic peroxides and hydroperoxides are the first reaction products between cellular constituents and free radicals or other reactive oxygen derivates. The Biomedica OxyStat assay measures the total peroxide concentration of a given sample. Results show a direct correlation between free radicals and circulating biological peroxides and thus allow the characterization of the oxidative status in biological samples.

Assay characteristics:

Cat.No.: BI-5007
Method: Colorimetric assay, 12x8-well strips
Sample type: Serum, EDTA plasma, biological fluids
Standard range: 1-point calibration, 2 controls  
Sample size: 20 µl / well
Incubation time: 1.5 h / 1.5 h / 30 min

Sensitivity:
LOD: 0.05 µg/ml (0 µg/ml + 3 SD)

Precision:
Intra-assay (n=3) ≤ 8%, Inter-assay (n=1) ≤ 14%

Values from apparently healthy individuals:
Median = 372 µmol /l (serum < 350 µmol/l, EDTA-plasma <400 µmol/l) 
It is recommended to establish the normal range for each laboratory.   

Principle of the assay:
The peroxide concentration is determined by reaction of the biological peroxides with peroxidase and a subsequent colour-reaction using TMB as substrate. After addition of a stop solution, the coloured liquid is measured photometrically at 450 nm.

A calibrator is used to calculate the concentration of circulating biological peroxides in the sample (one-point calibration). 

 

• Download OXYSTAT's Protocol

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Resources