DetectX® Prostaglandin (PGE2 ) Multi-format Kits

 Arbor Assay's PGE2 EIA

• Scientific Relevane of Prostaglandin (PGE2)
• Application
• Arbor Assay's DetectX® Prostaglandin (PGE2 ) Multi-format Kits
• Resources
• Publications

Scientific Relevance of Prostaglandin (PGE2)

 PGE2

Eicosanoid signal transduction pathways are highly conserved and are involved in a number of physiological processes.

Prostaglandins are synthesized from arachidonic acid by cyclooxygenase (COX)‑1 or ‑2, which convert the acid into PGH2. This is further processed by cytosolic or microsomal prostaglandin synthases to become PGE2 or one of several other prostanoids. Prostacyclin is the major cyclooxygenase product in blood vessel walls and it is present in inflammatory
fluids in similar concentrations to PGE2. Prostacyclin is a potent vasodilator and is more potent than PGE2 in producing hyperalgesia.

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Application

 PGE2

PGE2 is produced by a wide variety of tissues and in several pathological conditions, including inflammation, arthritis, fever, tissue injury, endometriosis, and a variety of cancers. Other biological actions of PGE2 include vasodilation, modulation of sleep/wake cycles, and facilitation of human immunodeficiency virus replication. It elevates cAMP levels, stimulates bone resorption, and has thermoregulatory effects. It has been shown to be a regulator of sodium excretion and renal hemodynamics.

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DetectX® Prostaglandin (PGE2 ) Multi-format Kits

The DetectX® PGE2 EIA & CLIA Kits are designed to quantitatively measure PGE2 present in tissues, lysates, saliva, urine, plasma, and media.

A PGE2 standard is provided. Standards or samples are pipetted into a coated microtiter plate, and a PGE2-peroxidase conjugate is added. The binding reaction is initiated by the addition of a monoclonal antibody to PGE2. After incubation the plate is washed and substrate is added. The substrate reacts with the bound PGE2-peroxidase conjugate. For the EIA kits, the plate read at 450 nm. For the CLIA kits, luminescent signal is read after 5 minutes.

  • USE: Measure PGE2 without extractions
  • SAMPLE TYPE: Saliva, Urine, Serum, Plasma, Media
  • VERSATILE: Works directly with Mouse and Rat Serum
  • TIME TO ANSWER:
    High Sensitivity EIA - Overnight
    CLIA - Overnight
    Regular EIA - 2.5 Hours
  • SAMPLES/KIT:
    EIA - 40 or 232 in Duplicate
    CLIA - 39 or 231 in Duplicate
Item Catalog # Download
PGE2 Multi-Format EIA Kit, 1 Plate K051-H1 Product Manual
PGE2 Multi-Format EIA Kit, 5 Plate K051-H5 MSDS

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Resources

• Eicosanoid Sample Extraction Protocol
• Arbor Assays Kit Plate Dimensions


myassays

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Publications

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