ROS Brite™: For Reactive Oxygen Species Detection

ros brite banner

About Reactive Oxygen Species (ROS)

Reactive oxygen species (ROS) are chemically reactive molecules containing oxygen (such as superoxide, hydroxyl radical, singlet oxygen and peroxides). ROS is highly reactive due to the presence of unpaired valence shell electrons. ROS forms as a natural byproduct of the normal metabolism of oxygen and have important roles in cell signaling and homeostasis. However, during times of environmental stress (e.g., UV or heat exposure), ROS levels can increase dramatically. This may result in significant damage to cell structures. Cumulatively, this is known as oxidative stress. ROS are also generated by exogenous sources such as ionizing radiation.

Under conditions of oxidative stress, ROS production is dramatically increased, resulting in subsequent alteration of membrane lipids, proteins, and nucleic acids. Oxidative damage of these biomolecules is associated with aging as well as with a variety of pathological events, including atherosclerosis, carcinogenesis, ischemic reperfusion injury, and neurodegenerative disorders.

How ROS Brite™ works

ROS Brite™ reagents are a series new fluorogenic probes to measure oxidative stress in cells. The cell-permeant ROS Brite™ reagents are nonfluorescent and produce bright fluorescence upon ROS oxidation. The resulting fluorescence can be measured using fluorescence imaging, high-content imaging, microplate fluorometry, or flow cytometry. ROS Brite™ 570, 670 and 700 reagents have good selectivity to both hydroxyl radical and supoeroxide. ROS Brite™ 700 is optimized for in vivo imaging.

Product Cat # Ex/Em(nm) Assay Cell Permeability
ROS Brite™ 570 16000 556/566Cell-basedPermeable
ROS Brite™ 670 16002 650/666Cell-basedPermeable
ROS Brite™ 700 16004 680/706 In vivoNon-permeable

How Mag-Iso Works

Figure 1: Fluorescence spectra of ROS Brite™ 570?ROS Brite™ 670?ROS Brite™ 70 (in PBS solution of pH7.2)

Figure 2: ROS detected ROS Brite™

Link to AAT Bioquest page to view all the reactive oxygen species related kits

Contact us to get a quote today!