StellARray®qPCR Arrays from Bar Harbour BioTechnology

 BHB's Stellarray qPCR arrays

• Introduction of StellARray™ qPCR Arrays
• StellARray™ qPCR Arrays's workflow
• About Global Pattern Recognition™ (GPR)
• Resources

The StellARray™ Gene Expression makes gene-by-gene expression analysis, microarray data validation, biomarker discovery and siRNA knock-down verification simple, fast and reliable.

The increasing number of citations of the MIQE guidelines (minimum information for publication of quantitative real-time PCR experiments (Bustin et al. 2009) demonstrates a growing emphasis on standardized experimental practice for qPCR.

The SYBR®-based StellARray® qPCR Array System from Bar Harbour BioTechnology offers a simple and reliable system for gene expression analysis that meets the MIQE guidelines and makes it easier for qPCR users to compare and publish their results.

The StellARray™ qPCR Gene Expression system consists of the GeneSieve™ Query, StellARray™ qPCR Arrays, and the Global Pattern Recognition™ Analysis Tool, all of which will enable researchers to perform a bioinformatics search to select genes or pathways of interest, measure the gene expression or copy number of samples using Real-Time PCR arrays, and determine the statistical significance of data using our web-based analysis tool.

  • GeneSieve™ Query: Bioinformatics – Gene or biological pathway selection
  • StellARray™ qPCR Arrays: Real-Time PCR Arrays – SYBR® Green I based primers arranged in 96-well and 384-well formats for real-time PCR
  • Global Pattern Recognition™ Analysis Tool - Bioanalysis – Analyze data without housekeeping genes

 

 stellarray qPCR array components

StellARray™ qPCR Workflow

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StellARray™ qPCR Arrays: Over 150 "constellations"

Over 150 pre-validated pathway or disease area-specific qPCR array detect gene expression and gene copy number variation:

  • Predefined pathways for Human and Mouse.
  • Designed for use with cDNA or gDNA to hit all splice variants of target genes.
  • All primers are wet lab validated every time - data are visually inspected to ensure
    specificity and reproducibility.
  • Arrays can be used on most common real-time PCR instruments with any
    SYBR® Green master mix. Truly an open array platform.
  • Available for 96, 384, and 4 x 96 well formats.
  • Dried down format ready for use or stable for one year.
  • Built-in controls: 18S positive control (invariant highly expressed endogenous
    control) and genomic DNA control as a negative control to detect the presence of genomic DNA contamination.
  • Custom Arrays with any gene content from any species and on any plate of your
    choice are available.
  • StellARray™ qPCR array users can access the proprietary Global Pattern RecognitionTM (GPR) algorithm from The Jackson Laboratory for reliable data analysis.
 stellarray workflow

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About Global Pattern Recognition™ (GPR)

GPR Reveals REAL Results:
  • No assumption of invariant normalize.
  • Compares Ct values for each gene in each sample.
  • Normalizes data from within the experiment.
  • Returns a statical p-value.
  • Ranks genes by significance or the change regardless of fold change.
  • Can be used with or without standard housekeeping genes.

Below is an example of a GPR report:

 GPR report

 

How GPR works:
  • The patented algorithm eliminates the challenges associated with the definition of a “normalizer” gene or gene set by comparing the changes between closely matched biological replicates within an experiment and choosing the least changing genes
  • GPR takes the Real-Time PCR data from your run and generates a ranked hit list of statistically significant changes between the test groups without reliance on the magnitude of the fold-change.
  • This approach provides a true statistical analysis of results based on internal consistency of the data allowing GPR to define the invariant normalizer genes, not the researcher. The result is detection of small, reproducible changes in gene expression that often carry large biological significance.

 

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Resources

  • GPR Flyer (PDF)

 

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