25-OH Vitamin D ELISA

25oh vitamin D biorad


Calbiotech’s 25-OH Vitamin D ELISA features 100% Cross-reactivity to D2 & D3 and no outside extraction step! This is a sensitive, robust and automation-friendly assay, requiring no external sample preparation and no organic solvents with a 2.5 hour total assay time.

Clinical Significance

Vitamin D is a steroid hormone involved in the active intestinal absorption of calcium and in the regulation of its homeostasis. Vitamin D has two isomers: Vitamin D2 and Vitamin D3. Vitamin D2 is obtained from dairy products whereas Vitamin D3 is produced in the skin after exposure to ultraviolet light. In the liver, Vitamin D is hydroxylated at its carbon 25 to form 25-OH Vitamin D. This metabolite is the predominant circulating form of Vitamin D and is considered to be an accurate indicator of the general Vitamin D status of an individual. Vitamin D deficiency has been linked to many diseases including osteoporosis, rickets, osteomalacia, cancers, and cardiovascular diseases. Both dietary supplements of Vitamin D that are currently available in the market (Vitamin D2 and Vitamin D3) are converted to 25-OH Vitamin D in the liver. The sum of the concentrations of 25-OH Vitamin D2 and 25-OH Vitamin D3, in serum or plasma, is referred to as “Total 25-OH Vitamin D”. Accurate monitoring of total 25-OH Vitamin D level is critical in clinical settings. Vitamin D deficient patients who are prescribed a daily Vitamin D supplement should regularly monitor their serum or plasma Vitamin D levels in order to reach an optimal level and prevent their 25-OH Vitamin D concentrations from reaching excessive levels that are considered toxic.

Kit Features

• Wide dynamic range: 1.25ng/mL to 150ng/mL
• Reproducibility: intra and inter-precision <8%
• Linearity: 90% - 111%
• 100% cross-reactivity to D2 & D3
• Standards traceable to NIST SRM-972A
• Ease of use: No off-well sample extraction
• No organic solvents
• Sensitive, robust & automation-friendly
• 2.5 hour total assay time (90 minute CLIA)
• ELISA & CLIA formats

vitamin D ELISA workflow