Ames Test

ames test plate muta-chromoplate

OVERVIEW OF AMES TEST

The Muta-ChromoPlate kit is a convenient approach for the detection of mutagenic activity and mutagenic materials in chemical water, sediment, air, chemicals, food components, cosmetics and biological fluids. The Muta-ChromoPlate is a 96-well microplate version of the Salmonella typhimurium ‘Ames Test’, and provides a clear colour endpoint. Reagents, cultures and other consumable components are ready-to-use in a non-specialized laboratory.

The Muta-ChromoPlate kit is based on the most generally used and validated bacterial reverse-mutation test, known as the ‘Ames Test’ (Ames et al., 1975, Mutation Research 31: 347). The test employs a mutant strain, or several strains, of Salmonella typhimurium, carrying mutation(s) in the operon coding for histidine biosynthesis. When these bacteria are exposed to mutagenic agents, under certain conditions reverse mutation occurs conferring on the bacteria the ability to again produce the amino acid histidine.

The Muta-ChromoPlate™ is a 96-well microplate version of the salmonella typhimurium ‘Ames Test,’ used for detection of mutagenic activity.

Developed to test mutagenic materials in water soluble extracts of sediment, air, chemicals, food components, cosmetics, waste waters, portable waters and any other material that can be solubilized or placed into micro suspension in water, such that the material being tested can be taken up by the test strain.

The Muta-ChromoPlate™ provides a clear colour endpoint. Reagents, cultures and other consumable components are supplied ready-to-use in a non-specialized laboratory

  • 5051 Muta-ChromoPlate Basic Kit (Contains 1 bacterial, reagents, controls and plastics)
  • B5051 Muta-ChromoPlate Bacterial Strain Kit (Contains 2 bacterial reagents, controls and plastics)
  • 5051S9 Activation Enzymes (designed for the Ames Test)
muta-chromoplate ames test
ames test procedure explained

Blank Plate

To show that proper laboratory techniques and sterility techniques were used.

Background Plate

This is an indication of a rate of natural reverse mutation.

Unknown Sample

Showed a mutagenic response significantly above the background rate.

Positive Control

Plate of a known mutagenic material which is included with the kit.