Antibody Production

Chicken IgY Antibody Production Service

chicken igy antibody mbio Over our long experience in Chicken IgY Antibody Production Service for diverse customers in academia and the biotech, diagnostic and pharmaceutical industries, we have developed a number of proprietary methods and procedures that optimize the immune response of our host animals and improve the quality of our products.

Whereas most competitors work primarily with rabbits, our scientists work exclusively with chicken in Polyclonal Antibody Production. As a result, our scientists’ deep experience with chickens allows our customers to benefit from unique capabilities, among them improved algorithms for analyzing protein sequences to identify immunogenic peptides.

The most human way of raising antibodies for your research! For over two decades, MBIO's principal's lab has been providing custom chicken IgY antibody production for customers in academia and industry worldwide.


custom chicken igY antibody production

Animal-friendly

We purify the antibodies from eggs, not serum. With rabbits, in contrast, serum is obtained by restraining the animals, and performing ear bleeds or cardiac puncture. It is simply a more humane way to produce antibodies.

Higher titres against highly-conserved mammalian gene products

Since chickens are at least 100 million years removed from mammals, they tend to recognize any mammalian gene product as foreign and mount vigorous immune responses.

Higher signals

Chicken IgYs contain a larger glycosylation index, allowing more labeling with HRP and other antibody tags. This produces higher signals.

Easier double immunostaining

Chicken IgYs can be used together with mouse and rabbit antibodies, without the danger of cross-reactivity. Secondary antibodies against chicken IgYs don’t cross-react with mammalian IgGs.

Cost-effective and longer-lasting

Our chicken antibody preparations are >90% IgY, and have shelf-lives of 5 years or more at 4˚C. In contrast, rabbit serum (which is what most companies provide) has only a limited shelf-life at 4˚C (measured in weeks-to-months), and loses some biological activitites when frozen. The cost of rabbit antibody purification to comparable purities (i.e., protein A-purified) is very costly.

Nearly unlimited quantities

We collect about 18 “immune eggs” from each hen, but only use 6 “immune eggs” to prepare your IgY preparation. We then store the remaining 12 “immune eggs” in the refrigerator, in case you need more antibody down the road. Moreover, we can continue to house the hens (for an additional charge), in case you want us to perform additional injections. <?p>

No “Fc domain” within the stem portion of a chicken IgY

This provides several advantages over rabbit IgGs:

  • It reduces the likelihood of having false positives in diagnostic applications, since it is the mammalian Fc domain that binds “rheumatoid factors” or other naturally occurring anti-Fc antibodies.
  • Does not activate mammalian complement systems, allowing the use of chicken IgYs in in vivo applications.
  • Does not bind to mammalian Fc receptors, avoiding non-specific binding to cells expressing such receptors (e.g., macrophages and dendritic cells).

chicken igY vs igg

 

 

You can read more about the benefit of Chicken IgY antibody at the following publication here.


Chicken IgY Antibody Production Features

Support Projects of Any Size

Chicken IgY Antibody Production Service can require two hens or two hundred hens. That is why we designed our new facilities with flexibility in mind. As a result, we can tailor our custom antibodies service to suit your needs, including project sizes up to “bulk” production levels (>100mg).Contact us today »

Comprehensive Support and Service

Using our proprietary Immunogenicity Algorithm®, we provide complimentary peptide design help to identify the best sequences. For applications requiring recognition of native protein structure, such as immunohistochemistry, immunocytochemistry, and immunoprecipitation applications. We then perform all of the steps necessary for production of the final product. The steps include peptide synthesis, conjugation, antibody production and antibody purification.Contact us today »

Animal Friendly

In Chicken Polyclonal Antibody Production, we purify the antibodies from eggs, not serum. With rabbits, in contrast, serum is obtained by restraining the animals, and performing ear bleeds or cardiac puncture. As a result, Chicken IgY Antibody Production Service is simply a more humane way to produce antibodies.Contact us today »

Responsible Animal Treatment

In our Polyclonal Antibody Production, we purify the antibodies from eggs laid by the immunized hens. This method allows us to avoid animal restraints and bleeds. In fact, we never subject our hens to restraints, except briefly for the injections themselves.Contact us today »