Custom Polyclonal Antibody Production

Custom Polyclonal Antibody Production

Free antigen design for customized application-specific polyclonal antibody.

We provide customized services for producing application-specific polyclonal antibodies used in your research. Leverage our years of experiences in polyclonal antibody production: from antigen design, immunogen preparation, antibody development, antibody purification, modification, validation, to immunoassay development.

Highlights

  • Free Antigen Design – Based on experience, data, and characteristics of your desired target.

  • Wider Choice of Host Animals – Choose from SPF (specific-pathogen-free) host animals, including rabbit, mouse, rat, and guinea pig.
  • High Specificity – Antibodies are purified by Protein A and antigen affinity chromatography. When needed, ABclonal can provide cross-purification to obtain a more specific antibody.
  • Guaranteed Result – 1 ng of protein antigen or 100 ng of peptide antigen is detected when the antibody is diluted at 1:1000.

Polyclonal Antibody Production Process

polyclonal antibody production development service

Antigen Design

We immunize rabbits, mice, rats, and guinea pigs to generate custom polyclonal antibodies against peptide antigens and protein antigens. Both peptide and protein antigens can be provided by you or prepared by us.

  • High Specificity – Antibodies are purified by Protein A and antigen affinity chromatography. When needed, ABclonal can provide cross-purification to obtain a more specific antibody.
  • Guaranteed Result – 1 ng of protein antigen or 100 ng of peptide antigen is detected when the antibody is diluted at 1:1000.

Please provide more than 4 mg of protein antigen with purity above 80% and concentration above 1 mg/ml (immunization requires 3 mg and antigen affinity purification requires more than 4 mg of antigen); protein SDS-PAGE electrophoresis map.

Target gene sequence and plasmid templates (more than 20 µl at 100 ng/µl concentration). We can also synthesize the gene if templates are not available; constructed expression plasmid or expressed bacteriophage (expression conditions and protein SDS-PAGE electrophoresis map).

Please provide more than 10 mg of peptide antigen with purity above 85% (immunization requires 4 mg and antigen affinity purification requires more than 6 mg of antigen);HPLC and MS report.

Please provide UniProt ID or NCBI Accession Number of your target protein.

If a UniProt ID or NCBI Accession Number is not available, please provide the nucleotide sequence (From ATG to termination codon) and/or the amino acid sequence

Immunization Schedule

ABclonal’s standard protocol for polyclonal antibody is optimized to produce antiserum in the shortest possible time. It is the most affordable option and a popular choice among researchers.

The protocol uses two New Zealand White rabbits that are specific-pathogen-free. Injections are subcutaneous (SQ) as emulsions in Complete Freund’s Adjuvant (CFA) or Incomplete Freund’s Adjuvant (IFA). We guarantee that the same antigen will be injected into both animals.

  • High Specificity – Antibodies are purified by Protein A and antigen affinity chromatography. When needed, ABclonal can provide cross-purification to obtain a more specific antibody.
  • Guaranteed Result – 1 ng of protein antigen or 100 ng of peptide antigen is detected when the antibody is diluted at 1:1000.