DNA Damage

dna damage 8-ohdg

DNA damage caused by oxidative stress is frequently associated with carcinogenesis, neurological disorders, and cardiovascular disease.

Free radicals and reactive oxygen species (ROS) are produced during normal cellular metabolism and also by ultraviolet or ionizing radiation. Reactive species are continually generated in vivo and often cause oxidative damage to biomolecules like proteins and DNA. Oxidative damage is normally held in check by multiple antioxidant and repair systems, but excessive oxidative stress damages tissues, potentially leading to a number of diseases. It is widely thought that continuous oxidative damage to DNA is a significant contributor to the age-related development of major cancers such as colon, breast, rectum, and prostate.

Oxidative DNA damage is ordinarily repaired by cellular base excision repair mechanisms producing the 8-hydroxy-2’-deoxyguanosine product (8-OHdG). The formation 8-OHdG is commonly observed in systems under oxidative pressure. Quantitation of 8-OHdG is an accurate measurement of DNA damage. Therefore, the resulting 8-OHdG excreted without further metabolism into urine can be used as an oxidative marker marker.

guanosine ros rns

Background of 8-OHdG

8-hydroxy-2-deoxy Guanosine (8-OH-dG) is produced by the oxidative damage of DNA by reactive oxygen and nitrogen species. 8-OH-dG serves as an established marker of oxidative stress. 1-4 Hydroxylation of guanosine occurs in response to both normal metabolic processes and a variety of environmental factors (i.e., anything that increases reactive oxygen and nitrogen species). Aging process as well as a number of pathological conditions including cancer, diabetes and hypertension, associates with increased levels of 8-OH-dG. In complex samples such as plasma, cell lysates, and tissues, 8-OH-dG can exist as either the free nucleoside or incorporated in DNA. Once the blood enters the kidney, free 8-OH-dG is readily filtered into the urine, while larger DNA fragments remain in the bloodstream. Because of the complexity of plasma samples, urine is a more suitable matrix for the measurement of free 8-OH-dG than plasma.

DetectX® DNA Damage ELISA Kit

8-hydroxy-2-deoxy Guanosine (8-OH-dG) is produced by the oxidative damage of DNA by reactive oxygen and nitrogen species. 8-OH-dG serves as an established marker of oxidative stress. 1-4 Hydroxylation of guanosine occurs in response to both normal metabolic processes and a variety of environmental factors (i.e., anything that increases reactive oxygen and nitrogen species). Increased levels of 8-OH-dG are associated with the aging process as well as with a number of pathological conditions including cancer, diabetes, and hypertension. In complex samples such as plasma, cell lysates, and tissues, 8-OH-dG can exist as either the free nucleoside or incorporated in DNA. Once the blood enters the kidney, free 8-OH-dG is readily filtered into the urine, while larger DNA fragments remain in the bloodstream. Because of the complexity of plasma samples, urine is a more suitable matrix for the measurement of free 8-OH-dG than plasma.

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About Arbor Assays

The founders and top scientists from Arbor Assays have been researching and developing high quality assay kits since 1982. Arbor Assays grew up making FDA-regulated in vitro diagnostic products for human disease detection. When they started in 1991 they realised that sensitivity, speed, convenience, quality and service were not associated with life science products. They changed that for you.

On January 1, 2017, Arbor Assays became the world’s first Employee-Owned life sciences company. As an employee benefit trust (EBT), the board of directors ensures that the company remains an independent entity for the benefit of customers and employees.

For the customer, employee ownership means that the company can be counted on to continuously experiment, innovate, and provide high-quality products.

For employees, ownership means increased job security and career satisfaction without the fear that Arbor Assays will be sold out to a larger development company.

MBIOSCIENCE is the authorised distributor for Arbor Assays in Malaysia.