Lipid Peroxidation Assays

artherosclerosis oxidative stress ELISA
lipid peroxidation and oxidative stress

LIPID PEROXIDATION AND OXIDATIVE STRESS ELISA

Oxidatively modified lipoproteins (oxLDLs) play an important role in the progression of atherosclerosis and coronary artery disease. Accumulation of oxLDL in macrophages and smooth muscle cells causes foam cell formation, an initial step in the disease. Low-density lipoprotein (LDL), the main carrier of plasma cholesterol, consists of a hydrophobic core and a surface monolayer of polar lipids and Apolipoprotein-B (ApoB). Oxidative stress and the consequent formation of free radicals lead to the peroxidation of ApoB.

oLAB ELISA (BI-20032) for Determination of Autoantibodies against Oxidized LDL

Oxidized low density lipoprotein (oxLDL) is believed to play a critical role in the development and progression of atherosclerosis. Accumulation of oxLDL in macrophages and smooth muscle cells causes foam cell formation, an initial step in the disease. Autoantibodies against oxidatively modified LDL can be used as a parameter that consistently mirrors the occurrence of oxidation processes taking place in vivo. In fact, elevated levels of autoantibodies against oxLDL have been detected in the blood stream of patients with coronary artery disease. Moreover, recent studies indicate a correlation between autoantibodies against oxLDL and the progression of carotid atherosclerosis. Increased serum concentrations of oLAB have also been described in various diseases such as pre-eclampsia and systemic lupus erythematosus. Decreased oLAB titers were observed during septicemia and myocardial infarction.
An overview on the clinical applications of oLAB has been published.

OxyStat Assay (BI-5007) Determination of Peroxides in biological fluids

Cells and tissues are sensitive to oxidative stress, caused by the formation of free radicals. If not deactivated by antioxidants, organic peroxides and hydroperoxides are the first reaction products between cellular constituents and free radicals or other reactive oxygen derivates.

The determination of the oxidative status / oxidative stress is essential in today’s medical research and diagnostics. Methods used so far were either expensive (HPLC), or detected only degradation products of polyunsaturated fatty acids, like TBARS (thiobarbituric acid reactive substances).

The Biomedica OxyStat assay measures the total peroxide concentration of a sample, utilizing a quick and simple assay procedure. Results show a direct correlation between free radicals and circulating biological peroxides and thus allow the characterization of the oxidative status in biological samples.

MDA oxidative damage

MDA-oxLDL ELISA (BI-20022) for Determination of Oxidized LDL

Malondialdehyde (MDA) has been identified as one of the major lipid peroxidation products of LDL, thus playing an important role in the LDL oxidation.

The Biomedica MDAoxLDL ELISA specifically detects MDA-modified ApoB in human serum and citrate-plasma.