Pre-filled Mgpure Viral RNA Purification Kit

Prefilled, pre-plated plates with magnetic beads for extraction of viral RNA from virus infected specimen (e.g. COVID-19) suitably configured for KingFisher Flex and Allsheng Autopure 96 purification systems.

ACCEPTED SPECIMENS

Respiratory specimens including: nasopharyngeal or oropharyngeal aspirates or washes, nasopharyngeal or oropharyngeal swabs, broncheoalveolar lavage, tracheal aspirates, and sputum.

Swab specimens should be collected only on swabs with a synthetic tip with aluminum or plastic shafts. Swabs with calcium alginate or cotton tips with wooden shafts are not acceptable

BRIEF PROTOCOL

1. Add 400 µL Buffer LYE to 200 µL sample. Mix well by vortexting for 5 seconds.
2. Add 10 µL MgPure beads to the sample, mix well by pipetting and incubate at room temperature for 5 min.
3. Place the sample tube on the magnetic rack to magnetize the MgPure beads until the beads are completely cleared from the solution.
4. Remove the clear solution from the beads. Take the sample tube off the magnetic rack and resuspend the beads with 500 µL Buffer RB. Place the sample tube on the magnetic rack to magnetize the beads, remove the clear solution when the beads are completely cleared from the solution.
5. Take the sample tube from the magnetic rack and resuspend the beads with 600 µL RNA Wash Buffer. Place the sample tube on the magnetic rack to magnetize the beads, remove the clear solution when the beads are completely cleared from the solution.
6. Air-dry the sample for 5-10 minutes. Take the sample tube from the magnetic rack and resuspend the beads with 15-30 µL DEPC-treated H2O. Place the sample tube on the magnetic rack to magnetize the beads, transfer the clear solution to a new tube when the beads are completely cleared from the solution. Store RNA at -80℃.