SCIVAX 3D NanoCulture® Plate

SCIVAX's Nanoculture Plate by MBIOBIOSCIENCE

• What is 3D Cell Culture?
• Types of 3D Cell Culture
• SCIVAX's NanoCulture® Plate and Dish
• How NanoCulture® Plate works?
• Advantages of NanoCulture® Plate/Dish
• Format Available
• Resources

What is 3D Cell Culture?

diaplex workflow by MBIOSCIENCE

In vivo, cells exist in three-dimensional aggregate/mass, and interface with the other cell or extra cellular matrix (ECM). In conventional in vitro cell culture method which is easy and has a long history, the cells attach to culture dish and form monolayer. This method have achieved many knowledge in biology. However, it is reported many times that the cells cultured in 2D method are far different from the cells in vivo. The excessive activation of proliferation signal caused by cells attachment to the plastic dish and poor (two-dimensional) interface with the other cells are the main reasons.

Recently, it is well known that cells grown under 3D cell culture technique more closely mimic natural tissues and organs microenvironment than cells grown in 2D. In 3D cell culture, cells form multi-cellular clusters (spheroids) that are driven by cell-cell and cell-matrix contracts, rather than cell-plastic contracts. In this environment, cells can exert forces on one another and can move and migrate as they do in vivo.

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Types of 3D Cell Culture

3D cell culture methods fall into two major groups. One is culturing in hydrogel sponge, e.g. ECM and agarose (i.e. scaffold type). Another is culturing in the low attachment dish (i.e. scaffold-free type). These methods have merit and demerit, and these are used according to the situation demand.


Types of 3D Cell Culture
  Scaffold Type Scaffold-Free Type
  • Has many past records and continuousness in research (standard method for 3D culture)
  • Cells cultured in this method mimic in vivo atmosphere, and have a high proliferative character
  • Suitable for construction of structural tissue
  • Many Plate-type products, which can be handled like conventional 2D culture plate, are available
  • Plastic products with high uniformity
  • Suitable for HTS
  • Scaffold made with biological product has problem in the stability and reproducibility.
  • Cumbersome procedure (coating and/or icing).
  • Not suitable for high throughput observation, because spheroids exist in different depth in the scaffold.
  • Cells proliferation under this condition is low, because proliferation signal from cell adhesion molecule is poor.
  • Cells form spheroid engulfing dead cells, and/or a lot of cells die in the spheroid because of low nutrients and low oxygen .

Scaffold-based 3D cell culture biological products are usually using for basic research like cancer, regenerative medical and ADME/Tox. However, it is recognized that this method is not suitable for experiments/tests, which need a high reproducibility and throughput, such as drug screening method, because of lot-to-lot variation and cumbersome procedure.

SCIVAX's NanoCulture® Plate and NanoCulture Dish, is the scaffold type 3D culture system without any hydrogels or chemical pre-treatments. It is a nice combo of both type. It can be handled like conventional 2D culture plate but cells cultured on NCP have high proliferative character, high uniformity and suitable for HTS.

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SCIVAX's NanoCulture® Plate and Dish?

SCIVAX's Nanoculture plate now available from MBIOSCIENCE

SCIVAX Life Sciences is a leading 3D cell culture technology company based in Japan. With their bio-mimetic, surface microprocessing technology, they culture multi-celluar spheroids on a film with an ECM mimicking structure, which can
simulate an in vivo-like micro-enviroment. Microplate format products using their technology are available on the market now, sold as NanoCulture® Plate (NCP).

NanoCulture® Plate (NCP) and NanoCulture® Dish (NCD) are engineered with micro-patterned structure on a special plastic film on the well-bottom surface. This pattern act as a scaffold for cells, and support cells to form cell spheroids. This nano-scale structure control the minimum attachment of cells to the film and enhances cells migration and proliferation while cells form spheroids.

spheroid formation on Nanoculture plate by MBIOSCIENCE


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How NanoCulture® Plate works?


spheroid formation on Nanoculture plate by MBIOSCIENCE

Cells seeded on NCP/NCD grip and attach to the nano-scale pattern on culture surface. Cells start migrating randomly and dynamically because these do not adhere strongly to the base film due to micro-structure, unlike conventional monolayer cell culture. Cells contact and adhere to each other while migrating on the surface, and form spheroids accompanied by proliferation at the same time. Spheroid growth reaches a plateau as culture period progresses. These cellular behavior is common through the 3D cell culture system using hydrogel or any other gel based matrix.

 HT29 spheroid formation on Nanoculture plate by MBIOSCIENCE

Spheroid Formation Process of BT474 Cell Line (Youtube Video)

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Advantages of NanoCulture® Plate & Dish

Ready-to-Use Same as conventional monolayer culture plates. After opening the package, just add cell suspension into wells.
Good Microscopic Observation Capable of parfocal observation for all spheroids within the view field. Can be measured directly with fluorescence/luminescence plate readers.
Uniform Production Lot Coating reagents free. No biological materials. Made of plastic material, with almost no lot-to-lot variation.
High Cell Viability Cells obtain high proliferation capacity by adhesion signal. Also, cells form spheroids while migrating, which cause highly viable cells attached to the plate form spheroids.
Rapid Spheroid Formation Capable of microscopic observation of spheroids by day 3 after seeding.
Large Scale Preparation of Spheroids 35mmφ dish with same system as plates is available. Capable of large scale preparation of spheroids with enlarged culture area.

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NanoCulture® Plate/Dish: Formats Available

NanoCulture Plate / Dish is serialized from 24, 96, 384-well plate to 35 mmφ dish. Variety of well sizes is applicable for different experiments, from HTS to large scale preparation of spheroids.

  35mm dish 24 well plate 96 well plate 384 well plate
Area (mm2) 754 193 34 10
Standard Seeding No. 200,00 cells/dish 60,000 cells/well 10,000 cells/well 3,000 cells/well
Extracted Total RNA (rough estimate after 1 week culture) 2-6 ug/dish 0.6-2 ug/well 0.1-0.3 ug/well 0.03-0.1ug/well
Recommended Use Protein/RNA Extraction

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